Elevated Concentrations of Free Fatty Acids (Linoleic Acids) May Inhibit Mesangial Cell Proliferation and Induce Cell Cycle Arrest and Apoptosis by Lipotoxicity

Rongguo Fu, Li Wang, Yan Du, Liqun Ma, Jie Gao, Zhao Chen, Ganglian Yao
2015 Hong Kong Journal of Nephrology  
Objective: To investigate the effects of 1a,25-dihydroxyvitamin D 3 [1,25(OH) 2 D 3 ] impact on the expression of Ki67 in human glomerular mesangial cells and its proliferation. Methods: Cultured human mesangial cells, taking the subcultured cells, which were randomly divided into four groups: normal control group; epidermal growth factor group (EGF group); 1,25(OH) 2 D 3 group; and combined group of EGF and 1,25(OH) 2 D 3 , treatment 48 h. The expression of Ki67 were detected by
more » ... d by immunofluorescene and fluorescence quantitative PCR (RT-PCR). Results: Compared with the normal control group, EGF group had a higher Ki67 expression (P < 0.05); Ki67 expression of 1,25(OH) 2 D 3 group was significantly reduced (P < 0.05); compared with EGF group, Ki67 expression in 1,25(OH) 2 D 3 group and combined group of EGF and 1,25(OH) 2 D 3 was low (P < 0.05), there was no significant difference between the normal control group and combined group of EGF and 1,25(OH) 2 D 3 (P > 0.05). Conclusion: 1,25-dihydroxyvitamin D 3 can inhibit the expression of Ki67 and the proliferation of human glomerular mesangial cells. http://dx.
doi:10.1016/j.hkjn.2015.09.120 fatcat:rmb4krx7vrc35lwccu63aj6n7y