The role of breathing exercise and their effect on a wide range of aspects relevant to orthopedics and sports rehabilitation has become the focus of a growing body of research, yet their effect in sport physical therapy is not well established. Objectives: To assess the effect of all fours belly breathing exercise on shuttle run performance among athletes. Methods: A total of 20 participants participated in this study. Group 1(n = 20) athletic group. Informed consent and study procedure was

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... ained to all the participants and a pre-shuttle run test (SRT) was assessed. After assessment the participants were engaged in all fours belly breathing exercise for 4 weeks and post SRT was assessed. Results: The mean SRT score before 4 weeks of all fours belly breathing exercise was 3.305. (Standard deviation = 1.46) The minimum score was 1.143 while the maximum score was 8.182. Whereas the mean SRT score after 4 weeks of all fours belly breathing exercise was 3.95 (Standard deviation = 1.36) The minimum score was 2.5 while the maximum score was 8.364. Whereas using student t test the mean difference between SRT score before 3.31 (1.47) and SRT score after 3.95 (1.37) is statistically significant (p<0.001). As well as the mean difference between predicted VO2max before 23.78 (4.90) and predicted VO2max after 25.87 (4.74) is statistically significant (p<0.001) Conclusion: Thus, this study concludes that all fours belly breathing is effective in improving the shuttle run performance among the normal subjects