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Fluorimetric Determination of Dichloroacetamide by RPLC with Postcolumn Detection
Bulletin of the Korean Chemical Society (Print)
An RPLC-postcolumn detection method has been developed for the fluorimetric determination of dichloroacetamide (DCAD) in water. After ammonia and DCAD were separated on a C 18 nonpolar stationary phase with 2.5% methanol-0.02 M phosphate buffer at pH 3, the column eluant was reacted with post column reagents, o-phthaldialdehyde (OPA) and sulfite ion at pH 11.5, to produce a highly fluorescent isoindole fluorophore, which was measured with a fluorescence detector (λ ex = 363 nm, λ em = 425 nm).doi:10.5012/bkcs.2004.25.6.900 fatcat:uegm4auphndofkg7afj7vbtvwa