FSHR (exon 10) gene polymorphisms and its association with fertility trait in Egyptian Ossimi sheep

Salah Abdel-Rahman, Yehia Mustafa, Hagar Errasool, Hanim Heikal, Ayaat Elmaghraby
2019 Biotechnology in Animal Husbandry  
For the association between of Follicle stimulating hormone receptor (FSHR) gene (partial part of exon 10) polymorphisms and litter size trait in Egyptian Ossimi sheep, polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and DNA sequencing techniques were developed. Fifty female Ossimi sheep reared under Egyptian conditions were selected according to their litter size. DNA from blood samples of these animals was isolated to amplify 250-bp of the FSHR gene
more » ... SHR gene influencing litter size production trait in sheep. Based on litter size, 50 animals were selected from the highest to the lowest litter size productivity during three seasons. PCR-SSCP analysis of the FSHR gene (250-bp) showed two various genotypes AA and AB with frequencies 0.64 and 0.36, respectively. The frequencies of the A and B alleles were 0.82 and 0.18, respectively.
doi:10.2298/bah1902127a fatcat:7o6qtbkhbveetnbcy3y4vkwrm4