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Indian Journal of Fundamental and Applied Life Sciences
A new simple colorimetric procedure is discovered for the estimation of retinal in egg yolk. This method does not require chromatographic separation of the other retinoids or carotenoids. After extraction by alcohol and petroleum ether, the petroleum ether part is evaporated to dryness by passing carbon-dioxide ; to it thiobarbituric acid reagent was added and mixed well. Immediately thiourea reagent was mixed and reading was taken after 30 minutes by a colorimeter at 530 nm. Calculation offatcat:wih5wwlc2vdeneeqfudrknotmm