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Replication of thermosensitive Rts1 plasmid deoxyribonucleic acid at the nonpermissive temperature
Journal of Bacteriology
Replication of the thermosensitive drug resistance factor Rtsl was studied at the nonpermissive temperature (42°C). It was concluded from the following observations that replication of this plasmid takes place at 420C without involving the covalently closed circular (CCC) form of deoxyribonucleic acid (DNA). (i) DNA-DNA reassociation kinetics studies with purified Rtsl DNA showed that Rtsl DNA increased several-fold during cell growth at 420C while very little, if any, CCC DNA was synthesized.doi:10.1128/jb.132.1.90-99.1977 fatcat:doxli7tqhbhyzhk73ib2e2xwzq