皿 isume c fotte , hr 黔 S へ ar ヒ d u ] arge increase in th1モ 皿 叮 団 曲 nd し 血 9 肌 〔in the り醜 r l d, 伽 傭 伽 mblebee > flight musclc 丘bers showed gruateT iV 〕 me 面 c 魚 alld a munh smaller lnen∋ase in 山 c TM 翻 . A 仁 the BL45XU bean丗 ne of SP 血 98 , we repeated exper ent5 with ve5 ' 廰 sh sp ∈ cimen6 ヱkコ thonコ zv . S and : tl 帥 eDnduebed expe 血 lents with (Zenaavmefis 〔 m ' . lnefly }n1 唇h匸 musOte 丘 bers . 厂 11he ム 勅 ( κvnLs ' expeli 皿 en お con 血rmedthepreviDus resul 儲 , d Lhe Ctt −J・ ltmb reb −iltS

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... s 皿ar to ・ those 。f 伽 わ 慨 . G っnveTgent ev 。 lu廿on y nou ve rreated exact thDpies げ asyndm 〕 nOusfligh niv ofScLence ;2 : JST, innov . P ]aan Isl コ i wa) Single skeletal myafibrls were 。 smotically compressed by Dex し [. an under-「a iousphySiologicnL conditions and the dhanges dfdiumeter and nsverse stfihess exami d byan atomi : f [ , rce n血 pe (AFM > . S噂e myo丘 b 画 1s were prepared by homog iz 血991yeerimtedmusclefibersot . 血 erabbiし pgaas , A 冊 . 玉 meagtuxtm 匸 mtSweieniadeaspre 、 us 且 y 且 〕 rm》℃ fibris attaChedtocover s ]ip . Modi 伽 dAFM 丗 ever91 弓 nga 蜘s田i . adasthe tip were used for me 闘 uremenLs . The diaIne 匸 ers彦 皿 d trmlsvcrse sti{thess of nglemyu 丘 bris respett.iiely deereased a【L(1increa $ edhy lixreasingthn Dcxtranooncentrat n up め16 %. The ・ diametEr ・ was l 、見 br副 axcd my 面 b曲 ,皿d 1. O pm fi 〕 r cantratti und or myり fibrilS . The dinmeter lfn 曜 ly d aB 甜 by ab〕 ut 15 % 島 r relaxed , rig andconn / acting mpUfibnls up tt 〕 2 % D 眠 tran. By fUrther ir皿ease ofDe 証 ti .a叫出 e diamet for or my σ且 b劃s did no 匸ch g 巳andimtfi ) r relaxedand contractingmyofibri ]sdem ℃a d byabcut 20% . Thet versest 曲 es5wasO .5 ,ユ、Oand L. 5pNfrim forrelaxed , enntrnc h ユ 目 drigor myofibr 丑s、 Up to 2 % Dextran , i しUnearly h1 as巳 d 3 . 4 time . for ngor myo 丘bn , and1 、5 . 2 口 me5 for relaxed nnd contra 面 dng m } u石 b 出 呂. By ber inen ∋ ase nf Den オr 跏, 止 e α ansver 蹴 丗 鰯 鯲 herinc 副 and 匸 hen [ eveledaffat6 '7衂 趾 詛 onbrMn 出 epm5ence of BDM, 血 e dimlleter m ユ d t vefse 5じ 冠 血ess 曲 ow 田 disbnoしcb ges by 血 crea 曲9 血 conDentra し ion . TTieresUltB 面 tained werere 夏 atedWith chang {n theattOmyO5infi ] a 皿 t oe血my ibr 皿5 . 一 S 59 一 N 工 工 一 Eleotronio