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Cell division, aging, and stress recovery triggers the spatial reorganization of cellular components in the cytoplasm, but also of membrane bound organelles, with molecular changes in their compositions and structures. However, it is not clear how these events are coordinated and how they integrate with regulation of molecular crowding. We use the budding yeast Saccharomyces cerevisiae as a model eukaryotic unicellular organism to study these questions using recent progress in opticaldoi:10.1101/2021.07.29.454334 fatcat:vzmd74hzijgp7nkf7fjj6frem4