The quantity and quality of methanogenic microorganisms based on rRNA analysis and their relation to methane production

Walczak Maciej, Swiontek Brzezinska Maria
2013 African Journal of Microbiology Research  
Biogas plants continuously convert biological wastes mainly into a mixture of methane, CO 2 and H 2 O a conversion that is carried out by a consortium of bacteria and archaea. The microbial community structure of the fermentation process was evaluated by using oligonucleotide probes complementary to conserved sequences of the 16S rRNAs of phylogenetically defined groups of methanogens. At the beginning of fermentation, Eubacteria are the main group and the count of Archaea then constitutes only
more » ... en constitutes only an insignificant percentage of the population of microorganisms. During the process of fermentation, we recorded a very significant increase in methanogenic microorganisms after 40 days of the process in progress. It is reflected in the quantity and the composition of released biogas.
doi:10.5897/ajmr12.1405 fatcat:p2itm5eahbdblig2qmszbvb54i