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expression vector (Invitrogen) were selected using Zeocin (0.5 mg/ml). To release soluble 7BP anchored on the cell surface, we treated cells with thrombin (10 IU/ml) for 3 days under serum-free conditions. The cleaved 6-His-and FLAG epitope-tagged recombinant 7BP in serum-free conditioned media was purified by sequential nickel and anti-FLAG affinity chromatography. For antibody production, 5 mg of purified 7BP emulsified in Freund's adjuvant were injected into rabbits (Strategic BioSolutionsdoi:10.1126/science.1067226 pmid:11809972 fatcat:dkl4n5nogbbrbms4f76q62zk7i