Multidetection of antibiotics in liver tissue by ultra-high-pressure-liquid-chromatography–tandem mass spectrometry

Andreia Freitas, Jorge Barbosa, Fernando Ramos
2015 Journal of chromatography. B  
Highlights: 2 3 4  The proposed method allows to determine 39 antibiotics in liver simultaneously; 5  Validation process shows the good performance of the method; 6  The present method is a huge improvement for laboratories involved in food 7 safety control. Abstract 37 38 A multiresidue quantitative screening method covering 39 antibiotics from 7 different 39 families by Ultra-High-Pressure-Liquid-Chromatography tandem Mass Spectrometry 40 (UHPLC-MS/MS) is described. Sulfonamides,
more » ... oprim, tetracyclines, macrolides, 41 quinolones, penicillins and chloramphenicol are simultaneously detected in liver tissue. 42 A simple sample treatment method consisting of extraction with a mixture of 43 acetonitrile and ethylenediaminetetraacetic acid (EDTA) followed by solid-phase 44 extraction (SPE) with a hydrophilic-lipophilic balanced (HLB) cartridge was developed. 45 The methodology was validated, in accordance with Decision 2002/657/EC, by 46 evaluating the following required parameters: decision limit (CCα), detection capability 47 (CCβ), specificity, repeatability and reproducibility. The precision, in terms of the 48 relative standard deviation, was under 22% for all of the compounds, and the recoveries 49 were between 80% and 110%. The CCα and CCβ were determined according to the 50 maximum residue limit (MRL) or the minimum required performance limit (MRPL), 51 when established. 52 53 54
doi:10.1016/j.jchromb.2014.11.008 pmid:25481418 fatcat:6taueax2wfenvbokuii6u4xotq