Characterization of the temperate bacteriophage phi adh and plasmid transduction in Lactobacillus acidophilus ADH

R R Raya, E G Kleeman, J B Luchansky, T R Klaenhammer
1989 Applied and Environmental Microbiology  
Lactobacillus acidophilus ADH is lysogenic and harbors an inducible prophage, +adh. Bacteriophage were detected in cell lysates induced by treatment with mitomycin C or UV light. Electron microscopy of lysates revealed phage particles with a hexagonal head (62 nm) and a long, noncontractile, flexible tail (398 nm) ending in at last five short fibers. Phage 4adh was classified within Bradley's B1 phage group and the Siphoviridae family. The 4adh genome is a linear double-stranded DNA molecule of
more » ... ded DNA molecule of 41.7 kilobase pairs with cohesive ends: a physical map of the 4adh genome was constructed. A prophage-cured derivative of strain ADH, designated NCK102, was isolated from cells that survived UV exposure. NCK102 did not exhibit mitomycin C-induced lysis, but broth cultures lysed upon addition of phage. Phage 4adh produced clear plaques on NCK102 in media containing 10 mM CaC12 at pH values between 5.2 and 5.5. A relysogenized derivative (NCK103) of NCK102 was isolated that exhibited mitomycin C-induced lysis and superinfection immunity to phage 4adh.
doi:10.1128/aem.55.9.2206-2213.1989 fatcat:xd254x2lcjgvzcayypx7sxzleq