Nanofluidic preconcentration and detection of nanoparticles J. Appl. Phys. 112, 014304 (2012) Biofunctionalized AlGaN/GaN high electron mobility transistor for DNA hybridization detection Appl. Phys. Lett. 100, 232109 (2012) Polymer translocation under time-dependent driving forces: Resonant activation induced by attractive polymerpore interactions JCP: BioChem. Phys. 6, 05B620 (2012) Polymer translocation under time-dependent driving forces: Resonant activation induced by attractive

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... interactions We demonstrate the use of a highly effective biosensor array to fulfill the requirements of high intensity, reduced nonspecific adsorption (NSA), and low sample usage. The mixed self-assembled monolayers (SAMs), consisting of methyl-terminated and methoxy-(polyethylene glycol (PEG))-terminated silanes, were newly applied as the background layer to reduce the background NSA via wettability control. The surface was modified by a plasma process with a pattern mask. Gold nanoparticles (AuNPs) were grafted within pattern-modified regions to increase intensity and were modified with protein G variants with cysteine residues to immobilize the antibody proteins directly. The target protein samples were selectively dewetted by the high throughput wiping process, while retaining semi-contact with the substrate. The data revealed that the background NSA was significantly reduced by 78% with selective dewetting compared to the standard method. Furthermore, the peak intensity was improved 5 times by applying AuNPs as compared to that of a planar surface, and the protein requirement was significantly reduced versus the standard process.