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Hepatocytes were isolated from adult rat liver by perfusing with collagenase. Isolated hepatocytes were entrapped within various materials and entrapped hepatocytes were examined on abilities of ammonia removal and urea synthesis. Collagen, fibrin, Ca-alginate, K-carrageenan, agar, low gelling point agarose, urethanprepolymer (PU-3, PU-6), and photopolymerizing resin (ENT-4000) were used for entrapping materials. Hepatocytes entrapped within collagen, fibrin, and Ca-alginate showed highdoi:10.11392/jsao1972.14.249 fatcat:exn2ejfxtndvneac5ks3hp6isa