Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

Hideki Itoh, Satoshi Arai, Thankiah Sudhaharan, Sung-Chan Lee, Young-Tae Chang, Shin'ichi Ishiwata, Madoka Suzuki, E. Birgitte Lane
2016 Chemical Communications  
FLIM of ER thermo yellow and non-targeted mCherry reveals the Ca2+-dependent heat production localized to SR in C2C12 myotube.
doi:10.1039/c5cc09943a pmid:26977478 fatcat:oswpg2zrwzd2hl55mifqi6ahmu