Identification of L Forms by Polyacrylamide-Gel Electrophoresis

Theodore S. Theodore, James R. King, Roger M. Cole
1969 Journal of Bacteriology  
Crude membranes were obtained from L forms by allowing the cells to lyse in distilled water. The crude membranes were washed several times in distilled water, lyophilized, and extracted with phenol-acetic acid-water. The membrane proteins were separated electrophoretically in polyacrylamide gels run at pH 4.5. Electrophoretic patterns and densitometric tracings of the gels showed distinct, reproducible intergeneric differences among L forms of Proteus, Streptobacillus, Staphylococcus, and
more » ... ococcus. Differences within a genus could not be detected except between the group A streptococcal L forms and the one group D F-24 L form. This electrophoretic method offers possibilities for ready identification of L forms through the use of standard reference strains.
doi:10.1128/jb.97.2.495-499.1969 fatcat:ggmmkld2grhpfh52lzwm667aru