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Identification of L Forms by Polyacrylamide-Gel Electrophoresis
Journal of Bacteriology
Crude membranes were obtained from L forms by allowing the cells to lyse in distilled water. The crude membranes were washed several times in distilled water, lyophilized, and extracted with phenol-acetic acid-water. The membrane proteins were separated electrophoretically in polyacrylamide gels run at pH 4.5. Electrophoretic patterns and densitometric tracings of the gels showed distinct, reproducible intergeneric differences among L forms of Proteus, Streptobacillus, Staphylococcus, anddoi:10.1128/jb.97.2.495-499.1969 fatcat:ggmmkld2grhpfh52lzwm667aru