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Bitter Gourd or bitter melon (Momordica Charantia L.) is a common type of vegetable and safe for daily consumption. The seeds are part of bitter gourd that useless. Research on bitter melon seed oil has promising commercial applications. This study aims to determine the antioxidant potential and safety of bitter melon seed oil through acute toxicity study. The content of phenols, flavonoids, and antioxidant activity was analyzed. The antioxidant activity was evaluated using the DPPH method.doi:10.22146/mot.52743 doaj:1fac8a7ccb4f4c41bb35166a84bc9c5c fatcat:htrsg6sqorew5pbt7vulfqndae