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| Bovine brucellosis causes heavy losses in dairy animals. The current study was conducted to observe the prevalence of brucellosis in district Hyderabad, Pakistan. A total of Four hundred and Two (n=402) milk samples were collected from 30 towns during winter and summer seasons of 2014-2015 from Kundhi buffaloes. In winter, milk specimens (n=124) were collected from 12 towns and in summer (n=278) from 18 towns arbitrarily. All samples were investigated for Brucella abortus (B. abortus)doi:10.14737/journal.jahp/2016/22.214.171.124 fatcat:wpuoxhej4zbpxh3q42r463vrle