| Bovine brucellosis causes heavy losses in dairy animals. The current study was conducted to observe the prevalence of brucellosis in district Hyderabad, Pakistan. A total of Four hundred and Two (n=402) milk samples were collected from 30 towns during winter and summer seasons of 2014-2015 from Kundhi buffaloes. In winter, milk specimens (n=124) were collected from 12 towns and in summer (n=278) from 18 towns arbitrarily. All samples were investigated for Brucella abortus (B. abortus)

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... es using by milk ring test (MRT). An overall 1.99 percent (8/402) positive prevalence of B. abortus antibodies was detected in milk of Kundhi buffalo. From 124 milk samples collected throughout winter only 3 (2.41%) were found positive while, among 278 milk samples taken throughout summer, 5 (1.79%) were declared as positive. It was concluded that prevalence of brucellosis in Kundhi buffaloes was low in Hyderabad, Pakistan.