Structure of a Peptidoglycan Amidase Effector Targeted to Gram-Negative Bacteria by the Type VI Secretion System

Seemay Chou, Nhat Khai Bui, Alistair B. Russell, Katrina W. Lexa, Taylor E. Gardiner, Michele LeRoux, Waldemar Vollmer, Joseph D. Mougous
<span title="">2012</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/5ihveavyobfs3mlzee7cjt7mfe" style="color: black;">Cell Reports</a> </i> &nbsp;
The target range of a bacterial secretion system can be defined by effector substrate specificity or by the efficacy of effector delivery. Here, we report the crystal structure of Tse1, a type VI secretion (T6S) bacteriolytic amidase effector from Pseudomonas aeruginosa. Consistent with its role as a toxin, Tse1 has a more accessible active site than related housekeeping enzymes. The activity of Tse1 against isolated peptidoglycan shows its capacity to act broadly against Gram-negative bacteria
more &raquo; ... and even certain Gram-positive species. Studies with intact cells indicate that Gram-positive bacteria can remain vulnerable to Tse1 despite cell wall modifications. However, interbacterial competition studies demonstrate that Tse1-dependent lysis is restricted to Gram-negative targets. We propose that the previously observed specificity for T6S against Gramnegative bacteria is a consequence of high local effector concentration achieved by T6S-dependent targeting to its site of action rather than inherent effector substrate specificity.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/j.celrep.2012.05.016">doi:10.1016/j.celrep.2012.05.016</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/22813741">pmid:22813741</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC3401384/">pmcid:PMC3401384</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/pvtqvyo4pbhubdj72lwp2mpuvm">fatcat:pvtqvyo4pbhubdj72lwp2mpuvm</a> </span>
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