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Effects of gonadotropins and insulin-like growth factor-I (IGF-I) and IGF-II on steroid production by bovine granulosa cells in vitro
A serum-free culture system for bovine granulosa cells was developed. Effects of follicle-stimulating hormone (FSH), luteinizing hormone (LH), insulin-like growth factor-I (IGF-I), and insulin-like growth factor-II (IGF-II) on steroid production by bovine granulosa cells were determined. Bovine granulosa cells from small (< 5 mm), medium (6-10 mm), and large (>10 mm) follicles were cultured for 48 h. Concentrations of estradiol-17p (E₂) and progesterone (P₄) were measured by radioimmunoassay.doi:10.14288/1.0087227 fatcat:gpzfkwkbzbcgzptbeyzhofbini