Differential Expression of Lymphocyte Function-Associated Antigen 1(LFA-1) on Epidermotropic and Non-Epidermotropic T-Cell Clones

M.D. Tetsuo Shiohara, B.S. Noriko Moriya, B.S. Chie Gotoh, M.D. Jun Hayakawa, M.D. Kaj Saizawa, Hideo Yagita, M.D. Masaji Nagashima
1989 Journal of Investigative Dermatology  
Various T -ce U surface molecules are involved in T-cell adh esion. which is an essential req uirement for epidermo rropic migration ofT cells. OUf previous studies demonstra ted thar certain T-cell clones can migrate inco the epidermis upon their intradermal inoculation in to the foo tpads of recipient mice with relevant antigens. while other T-cell clo nes, despi re [heir identical an tigen specificities and fun c tions, are non-epidermorropic. W e therefore tested whether rh e differences io
more » ... epidermo tropism of th ese T ce ll s could reside in rhe differen r levels of expression ofT-ceil surface molecules such as CD3, CD4, C02, and lymphocyte function associated anrigen 1 (LFA 1). The results of flow cytometric analysis showed that LFA-l was preferenrially expressed on the surface of epidermotropic T-cell clones, while non-epidermotropic T-ce ll clones were negative or very w ea kl y posi-I 11 various skin dise.ases where T -ceU-mediated mechanisms are tboughr to be involved. T cd Is show a specinc affini ty for th e epidermis. known as epidc.rmorropisnl [1] . Although lymphocyte migration into lymphoid tissue has been extensively studied and the receptor-ligand type of interacrion between lymphocytes and postcapillary high endotheUal ven ules (HEV) has been proved to be essennal for the abiliry to migrate into lymphatic tissue 12J, much less is know n about the factors that govern T-cell t rafficking in th e skin . Most of the recent studies nave suggested [har mi gration of lymphocytes imo the epidermis may M:musc.npt rcccil/ed Muc.h 2. 1989; OIccepted for public3tion June 5,
doi:10.1111/1523-1747.ep12284430 pmid:2685124 fatcat:a37tmjx6qvghbmly27tve7yvwe