AGROBACTERIUM-MEDIATED GENE TRANSFORMATION OF POLLEN SPECIFIC GENE STK1 IN MAIZE (ZEA MAYS)
Applied Ecology and Environmental Research
Javeed et al.: Agrobacterium-mediated gene transformation of pollen specific gene STK1 in maize (Zea mays) -11789 -APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 17(5):11789-11802. Abstract. Maize is a major food, feed and fodder crop, grown extensively all over the world. Genetic engineering has a unique role in crop improvement. Serine-Threonine-Kinase1 (ZmSTK1) is a gene-based enzyme, which due to its biochemical action in maize pollen grains, lowers the survival capability and affects
... d affects pollination. Out of different methods of genes transfer into the genome of the target cell, Agrobacterium-mediated gene transformation is an easy and convenient method in which required gene is insert into the Agrobacterium, which after multiplication carry foreign genes into the host cell's genome. In this research, a simple and easy Agrobacterium tumefaciens-mediated method used to establish the genetic transformation system of zmstk1 into maize germinating embryo as receptor system. Transgenic plants obtained successfully through this method and the complicated culture process not needed. The expression of zmstk1 in the tissue was determined by observing the histochemical localization of zmstk1 in order to elucidate the expression of zmstk1 in mature tassel or pollen.