Mechanism of peroxidase actions for salicylic acid-induced generation of active oxygen species and an increase in cytosolic calcium in tobacco cell suspension culture

T. Kawano
2000 Journal of Experimental Botany  
that superoxide was converted to H 2 O 2 . In addition, it was observed that superoxide dismutase-insensitive Extracellularly secreted peroxidases in cell suspension ESR signal of monodehydroascorbate radical was culture of tobacco (Nicotiana tabacum L. cv. Bright induced by SA both in the tobacco suspension culture Yellow-2, cell line BY-2) catalyse the salicylic acid (SA)and HRP reaction mixture, suggesting that SA free dependent formation of active oxygen species (AOS) radicals, highly
more » ... icals, highly reactive against ascorbate, were which, in turn, triggers an increase in cytosolic Ca2+ formed by peroxidase-catalysed reactions. The formaconcentration. Addition of horseradish peroxidase tion of SA free radicals may lead to subsequent mono-(HRP) to tobacco cell suspension culture enhanced the valent reduction of O 2 to superoxide. SA-induced increase in cytosolic Ca2+ concentration, suggesting that HRP enhanced the production of AOS. The mechanism of peroxidase-catalysed generation of tabacum, peroxidase, salicylic acid, signal transduction. AOS in SA signalling was investigated with chemiluminescence sensitive to AOS and electron spin resonance (ESR) spectroscopy, using the cell suspension
doi:10.1093/jexbot/51.345.685 pmid:10938860 fatcat:hmt6xxtcw5dfzg5ge3xrfwpp44