Implementación del algoritmo reverso para el cribado de sífilis en un laboratorio clínico

M. Soledad Martínez-Methol, Fernando D. Ventimiglia, Ana M. Aristimuño, Amelia N. de la Colina, Jorge J. Bruno, Liliana E. D'Agostino
2019 Revista chilena de infectología  
Syphilis remains a public health concern worldwide, the accuracy of diagnostic tests is critical for its successful control. Currently, there are two approaches to the diagnosis of syphilis using serological tests: the traditional algorithm and the reverse algorithm. The goal of this study was to analyse the advantages and disadvantages in the implementation of the syphilis reverse-screening algorithm in an outpatient clinical laboratory. An observational cross-sectional study was carried out
more » ... y was carried out analyzing 246 reactive sera from a total of 14700 requests for syphilis serology. Chemiluminescent assay ARCHITECT Syphilis TP, V.D.R.L. and FTA-Abs were performed. Among 246 reactive sera by ARCHITECT Syphilis TP, 129 were reactive and 117 were non-reactive by V.D.R.L. the last mentioned resulted in 97 reactive and 20 non-reactive by FTA-Abs, suggesting false positives (0.13%). Two patients with primary infection were detected, that were not detected by V.D.R.L. and one pregnant woman with primary infection with a high value S/CO and V.D.R.L.:1 dils. Among the advantages of using a reverse algorithm were greater sensitivity in the detection of patients with primary syphilis; automation, complete traceability of the samples; objective interpretation and conclusive results.
doi:10.4067/s0716-10182019000400525 pmid:31859779 fatcat:wfiop2vkhvehfdfuogwhs3e3l4