An Evaluation of Vitek MS System for Rapid Identification of Bacterial Species in Positive Blood Culture

Kang-Gyun Park, Sang-Ha Kim, Jong-Tae Choi, Sunghyun Kim, Young-Kwon Kim, Young-Bin Yu
2017 Korean Journal of Clinical Laboratory Science  
1 가톨릭대학교 서울성모병원 진단검사의학과, 2 건양대학교병원 진단검사의학과, 3 경동대학교 임상병리학과, 4 부산가톨릭대학교 보건과학대학 임상병리학과, 5 건양대학교 의과학대학 임상병리학과 The aim of this study was to shorten the time required for subculture and bacterial identification and obtain a simple and rapid identification method for new test methods for bloodstream infections. The following results were obtained using a mass spectrometer. In Vitek 2, 208 (81.8%) cases were well-identified and 45 isolates were not identified in blood cultures. Among 208 cases, 146
more » ... g 208 cases, 146 (57.5%) were Gram positive bacteria and 108 (42.5%) were Gram negative bacteria. In total, 233 were identified to the species level and 21 were identified to the genus level. The identification error was found to be Propionibacterium acnes as Clostridium bifermentans. The accuracy of Enterobacteriaceae, glucose non-fermentative bacilli (GNFB), and staphylococci were 81/83 (97.6%), 12/15 (80.0%), and 72/85 (84.7%), respectively. The concordance rate of Vitek 2 and Vitek MS by the direct method was 81.8% and 45 isolates were not identified. Most of the unidentified bacteria were Gram positive bacteria (N=37). The Gram positive bacteria were streptococci (14), coagulase-negative staphylococci (CNS) (11), enterococci (3), Staphylococcus aureus (2), Micrococcus spp. (2), Bacillus spp. (2) and Actinomyces odontolyticus, Finegoldia magna, and Peptostreptococcus spp. The results reporting time was reduced to 24∼72 hours compared to the conventional method. The rate of identification of the aerobic and anaerobic cultures was similar, but the use of an anaerobic culture did not require a dissolution process, which could shorten the sample preparation time. These results suggest that the method of direct identification in blood cultures is very useful for the treatment of patients. In further studies, it might be necessary to further improve the method for identifying streptococci and CNS, which were lacking in accuracy in this study.
doi:10.15324/kjcls.2017.49.4.407 fatcat:jbyxivmygnbsbjmoj5huigjcwe