Possible Involvement of 5-Lipoxygenase Metabolite in Itch-Associated Response of Mosquito Allergy in Mice

Yasushi Kuraishi, Eiji Ohtsuka, Tasuku Nakano, Sanae Kawai, Tsugunobu Andoh, Hiroshi Nojima, Kiyoshi Kamimura
2007 Journal of Pharmacological Sciences  
This study investigated endogenous mediators involved in mosquito allergyassociated itching in mice. An intradermal injection of an extract of mosquito salivary gland elicited marked scratching in sensitized mice. The 5-lipoxygenase inhibitor zileuton (100 mg / kg), the 5-lipoxygenase activating peptide inhibitor MK-886 (10 mg / kg), and the glucocorticoid betamethasone 17-valerate (3 mg / kg) inhibited the scratching. The scratching was not affected by the cyclooxygenase inhibitors
more » ... and ketoprofen, the TP prostanoid receptor antagonist SQ-29548, the leukotriene B 4 antagonist ONO-4057, the cysteinyl leukotriene antagonist pranlucast, the leukotriene D 4 antagonist MK-571, the platelet-activating factor antagonist CV-3988, the nitric oxide synthase inhibitor N G -nitro-L-arginine methyl ester, the H 2 histamine-receptor antagonist cimetidine, the H 1 histamine-receptor antagonist terfenadine plus cimetidine, and cypoheptadine that blocks the 5-HT 1/ 2 serotonin receptors. Zileuton (100 mg / kg) inhibited the increased activity of the cutaneous nerve branch induced by an intradermal injection of the extract, suggesting the peripheral action. Zileuton and MK-886 (10 and 100 µM) did not affect high K + -induced increase in intracellular Ca 2+ concentration in cultured dorsal root ganglion neurons. The results suggest that 5-lipoxygenase metabolite(s) other than leukotriene B 4 and cysteinyl leukotrienes are involved in mosquito allergy-associated itching.
doi:10.1254/jphs.fp0070404 pmid:17827870 fatcat:n2ihlq35l5dvlfnnbocnkqq6mu