Objective: To evaluate the gender based comparison of blood pressure and heart rate in adolescent population. Study Design: Comparative Cross Sectional Study. Setting: Department of Physiology, Baqai Medical University. Period: 15 February to 15 August in 2017. Material & Methods: A total of 500 student of MBBS, BDS and DPT of Baqai medical university were enrolled in this study by non-probabilty, purposive sampling, after taking ethical approval. Blood pressure was measured by using

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... and mercury sphygmomanometer, which is applied to the arm. Heart rate is measured by palpating the radial artery. This collected data were analyzed by using SPSS version 22.0.This data of male and female adolescents were analyzed separately due to autonomic regulation. Results: It was observed that Gender based comparison of mean weight, mean body mass index (BMI), mean Systolic Blood Pressure, mean Diastolic Blood Pressure, and mean heart rate between males and females had statistically significant (p-value<0.05) differences. Among the categories of Diastolic Blood Pressure, only mean Normotensive blood pressure comparison in males and females showed statistically significant (p-value<0.05) difference, while Age, categories of systolic blood pressure (Normotensive, Prehypertension, Hypertension) and Diastolic blood pressure (Prehypertension, Hypertension) had showed statistically non-significant (p-value>0.05) gender difference in study participants. Conclusion: In our study results the mean BMI, mean Systolic Blood Pressure, mean Diastolic Blood Pressure, and mean heart rate had statistically significant (p-value<0.05) differences in gender.