Chromosome Territories Reposition During DNA Damage-Repair Response

Ishita S. Mehta, Mugdha Kulshreshtha, Sandeep Chakraborty, Ullas Kolthur-Seetharam, Basuthkar J. Rao
2014 Biophysical Journal  
Local higher order chromatin structure, dynamics and composition of the DNA are known to determine DSB frequencies and efficiency of repair. However, the effect of DNA damage response, vis-a-vis spatial organisation of chromosome territories is still unexplored. Our report investigates the effect of DNA damage on spatial organization of chromosome territories (CTs) within interphase nuclei of human cells. We show that DNA damage, in a dose dependent manner, induces a large-scale spatial
more » ... interior to periphery and vice versa) repositioning of CTs that are relatively gene dense. Further, we have found that CT repositioning is contingent upon DSB recognition and damage sensing. Importantly, our results suggest that this is a reversible process where following repair, CTs re-occupy positions similar to that in undamaged control cells. Thus, our report, for the first time highlights DNA damage dependent spatial reorganization of chromosomes, which might be an integral aspect of cellular damage response. 422-Pos Board B177 Structure and Mechanical Properties of the Bacterial Chromosome in E.Coli
doi:10.1016/j.bpj.2013.11.509 fatcat:rlqgkq6anffh3e3oisg2i3utae