Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds

Yousmel Alemán Gaínza, Rafaela Regina Fantatto, Francisco Celio Maia Chaves, Humberto Ribeiro Bizzo, Sérgio Novita Esteves, Ana Carolina de Souza Chagas
2016 Revista Brasileira de Parasitologia Veterinária  
The anthelminthic activity of the essential oil (EO) of Piper aduncum L. was tested in vitro on eggs and larvae of resistant (Embrapa2010) and susceptible (McMaster) isolates of Haemonchus contortus. The EO was obtained by steam distillation and its components identified by chromatography. EO concentrations of 12.5 to 0.02 mg/mL were used in the egg hatch test (EHT) and concentrations of 3.12 to 0.01 mg/mL in the larval development test (LDT). Inhibition concentrations (IC) were determined by
more » ... e SAS Probit procedure, and significant differences assessed by ANOVA followed by Tukey's test. In the EHT, the IC50 for the susceptible isolate was 5.72 mg/mL. In the LDT, the IC50 and IC90 were, respectively, 0.10 mg/mL and 0.34 mg/mL for the susceptible isolate, and 0.22 mg/mL and 0.51 mg/mL for the resistant isolate. The EO (dillapiole 76.2%) was highly efficacious on phase L1. Due to the higher ICs obtained for the resistant isolate, it was raised the hypothesis that dillapiole may have a mechanism of action that resembles those of other anthelmintic compounds. We further review and discuss studies, especially those conducted in Brazil, that quantified the major constituents of P. aduncum-derived EO.
doi:10.1590/s1984-29612016073 pmid:27925067 fatcat:kdcpijon5zgrxp2f5tkk7l5yeu