A study was conducted to assess the effect of estrus synchronization technique on fertility and estrus induction response in crossbred cows under field conditions. Totally 30 infertile cows (anoestrous 13 and repeat breeder 17) were selected and divided randomly into two groups having 15 cows each. Estrus synchronization protocol (Triu-B) was adopted in Group I and Group II cows were kept as control. All the cows (100%) under Triu-B protocol were exhibited estrus between 48 and 72 hours

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... g PG injection on day 7. Artificial insemination was carried out up to 3 subsequent estrus cycles in cows which were exhibited spontaneous or induced estrus. Number of inseminations, service period and pregnancy data were recorded and analysed statistically. In group I, mean number of service per conception was 1.42 whereas in group II was 2.43. The mean service period in days were differs significantly (P≤0.01) between treatment (129.42±4.73) and control (158.71±8.89) group. The higher conception rate (80 %) was obtained in treated group while in control group was 46.66 %. The results revealed that estrus synchronization protocol could be used effectively to augment reproductive performance in crossbred dairy cattle under field conditions.