Functional Significance of Prop-1 Gene Expression in Pituitary Adenomas

TAKESHI USUI, YOSHIO NAKAMURA, HARUO MIZUTA, HIROYUKI MURABE, SEIJI MURO, MICHIO SUDA, KIYOSHI TANAKA, ISSEI TANAKA, AKIRA SHIMATSU, KAZUWA NAKAO
2000 Endocrine journal  
Prophet of Pit-1(Prop-1), which is a paired-like homeodomain transcription factor, is capable of binding to sites in an early enhancer of the Pit-1 gene and regulating its expression. As human Pit-1 is expressed considerably in pituitary adenomas, we studied human Prop-1 gene expression in pituitary adenomas. We also sequenced the Prop-1 cDNAs in pituitary adenomas. Human Prop-1 transcript was detected in all pituitary adenomas examined by RT-PCR analysis. The expression of human Prop-1 in
more » ... uman Prop-1 in pituitary adenomas was confirmed by in situ hybridization in one of the GH-producing adenomas. The sequence analysis of human Prop-1 cDNAs in these pituitary adenomas revealed that there were no mutations except 5 silent nucleic acid substitutions, suggesting that mutations of Prop-1 gene do not represent a frequent mechanism of human pituitary tumorigenesis.
doi:10.1507/endocrj.47.supplmarch_s85 pmid:10890192 fatcat:y5drjho32jeeriel4gvae3wbim