Introduction The ultimate objective of winter-hardiness investigations is to find the fundamental nature of this phenomenon. Toward that end results are reported of a study on the autolysis of red clover root and crown tissue when in the cold-hardened and unhardened condition. Results are also reported from experiments on hardened red clover plants stored for 11 days at temperatures of 340, 310 and 120 F. Carbohydrate and nitrogen analyses were made on these tissues. It has been suggested (8,

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... that the sugars are related to protein precipitation, thus a study of the activity of the proteolytic and carbohydrate enzymes under similar conditions would appear to be of