Thin platinum working electrode deposits of chitosan − soaked carbon nanotubes ( CH 『 CNT > that were additionally charged with ferrocene ( Fc ) and glucose oxidase ( GOx ) as functional sensing elements have been explored as quantitative recognition Iayer fQr glucose biosensors . Demonstrated will be that the sophisticated Iayout of the estab 随 shed enzyme immobilization matrix allowed not only molecular O2 and FG as native and synthetic redox − active co − factors but also dlrect protein − to

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... − eIectrode electron transfer to repeatedIy work for oxidized GOx recove ry after enzymatic glucose conversion and tune the protein ' s catalytically aCtive site for non − stop interaction with continuously arriving sugar ta rgets . Motivation behind the use of the marine biopoiymer chitosan as part of the functional biosensor Iayer was the predictab [ e gain of a matrix biocompatibility supportive of GOx bng − term survival on the sensor sur 「 ace and hence analytical response stability . The stable and consistent manifestation of CH [ T℃ NT − Fc − GOx biosensor signals in a 刊ow − based electrochemical cell for the duration of day − long continuous exposures to adjusted glucose levels and success with on − line glucose calibration trials during unlnterrupted 刊ow cell operation wi [ 1 be reported as clearsigns that the immobHized enzyme entities fe[ t well enough in their polymeric chitosugarlCNT envi ronment to maintain biocatalytic activity for long, 応 用 糖 質 科 学 第 5 巻 第 3 号 ( 2015) N 工工 一 Electronlc Llbrary