Characterization of Four Novel CAG Repeat-Containing cDNAs

JIN-XING JIANG, RONALD H. LEKANNEDEPREZ, ELLEN C. ZWARTHOFF, PETER H.J. RIEGMAN
1995 Genomics  
the alternative triplets play a role in the stabiliza-Stretches of CAG nucleotides coding for the amino tion of the repeat (1). Only for SBMA, in which the acid glutamine are an important feature of many tran-CAG repeat in the gene encoding the androgen rescription factors and genes that are involved in neuroceptor is affected, is a clearcut function for the gene degenerative disorders. In an attempt to isolate CAG causing the disease known. However, since over 80% repeat-containing cDNAs
more » ... sed in nervous tissue, of the proteins containing glutamine stretches are we screened a human fetal brain cDNA library with a transcription factors (2) , it is most likely that this probe containing a CAG repeat. Five different clones will also be the case for at least some of the other were characterized and found to contain CAG repeats. genes. Sequence data revealed that four of these cDNAs were In an attempt to isolate additional genes conderived from novel genes. These cDNAs were desigtaining CAG repeats, we screened a human fetal nated CAG6, CAG12, CAG24, and CAG40 and were brain cDNA library (Stratagene 936206) with a 0.5found to correspond to transcripts of 5.0, 7.5, 4.4, and kb, CAG repeat-containing, PstI fragment from the 15 kb, respectively. The genes encoding CAG6, CAG12, human MN1 gene (6). From these five unique cDNAs CAG24, and CAG40 were assigned to chromosomes 12, were selected for further characterization. These 16, X, and 12, respectively. For the 5th gene, CAG26/ cDNAs were designated CAG6, CAG12, CAG24, pRHpA, a localization on two different chromosomes CAG26, and CAG40. The clones were sequenced over was established: 16 and X. None of the repeats showed the CAG repeat (see Fig. 1 ). Four of the five cDNAs any length polymorphisms in human DNA. ᭧ 1995 showed no significant homology apart from the re-Academic Press, Inc.
doi:10.1006/geno.1995.0015 pmid:8595911 fatcat:wrllqdxjzrdmjc2t2rltfdg35u