Original Article Expression of miR-204 and MMP-9 in Helicobacter pylori-associated gastric ulcer

Xuejun Li, Liping Wang, Gang Li, Xingfeng Zheng, Chonghao Duan
2016 Int J Clin Exp Med   unpublished
Aims: This study is to analyze the expression pattern of matrix metalloprotein 9 (MMP-9) in patients with Helicobacter pylori-associated gastritis and to investigate the molecular mechanism responsible for its regulation in H. pylori-associated gastritis. Methods: Ulcer tissues, blood and saliva samples were collected from 46 patients with H. pylori-associated gastritis. Normal stomach mucosa, blood and saliva in 29 healthy people confirmed by endos-copy biopsy were collected as control group.
more » ... as control group. The levels of MMP-9 mRNA expression were measured by quantitative Real-time PCR, and MMP-9 protein levels were analyzed by Western Blot and ELISA. MiRNAs that regulate MMP-9 expression was predicted by bioinformatics analysis, and the expression of predicted miRNA was measured by quantitative Real-time PCR. Results: MMP-9 was significantly upregulated at both mRNA and protein levels in ulcer tissues, blood and saliva samples from patients with H. pylori-associated gastritis compared with that from healthy control. MiR-204 was predicted to regulate MMP-9 expression, and miR-204 level was significantly downregulat-ed in samples from patients with H. pylori-associated gastritis. Conclusion: The miR-204 expression is decreased whereas the MMP-9 expression is increased in H. pylori-associated gastritis. MiR-204 might affect the healing of H. pylori-associated gastritis by regulating the expression of MMP-9, and therefore this miRNA could serve as a potential biomarker in the diagnosis of H. pylori-induced gastritis.