Isolation and Quantification of three Genes involved in the Expression of Anthocyanins in Rubus glaucus Benth by RT-qPCR

Viviana Chiluisa Utreras
2017 MOJ Food Processing & Technology  
The violet and purple colored fruits contain phenolic compounds specifically, anthocyanins. These have a high nutritional quality and anthocyanins are believed to have anti-aging, anti-carcinogenic and antioxidant properties in the fruit. Gene expression studies have demonstrated the importance of flavonoids and their biosynthetic pathways, further increasing the interests of research and sources. In the present study, for the isolation and quantification of the β-Actin, RuANS and RuMYB10 genes
more » ... S and RuMYB10 genes involved gene pathway induces anthocyanin Rubus glaucus Benth, the Real-Time Polymerase Chain Reaction Reverse transcription (RT-qPCR). According to the values obtained with the statistical analyzes DCA and the Tukey test at 95% confidence, observed a relationship between genes as no significant differences existed in the expression of the three, generating the following results: β Actin presented higher concentration (273.42 ng/mL), followed by the RuMYB10 gene (220.33 ng/mL) and finally the RuANS gene (212.75 ng/mL), concluding that the species studied had similar levels of expression three genes analyzed.
doi:10.15406/mojfpt.2017.05.00129 fatcat:puvzwi5qerempc4qjxzxw4guqy