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Identifying novel amino acid substitutions of hemagglutinin involved in virulence enhancement in H7N9 virus strains
2021
Virology Journal
Background To identify site-specific features of amino acid substitutions that confer enhanced H7N9 virulence in humans, we independently generated mammalian-adapted variants of A/Anhui/1/2013 (AH-H7N9) and A/Shanghai/2/2013 (SH-H7N9) by serial passaging in Madin-Darby canine kidney (MDCK) cells. Methods Virus was respectively extracted from cell culture supernatant and cells, and was absolutely quantified by using real-time polymerase chain reaction. Viral RNAs were extracted and subjected to
doi:10.1186/s12985-020-01464-1
pmid:33430903
fatcat:y5ovvuf5jjcbpnlkkw577bouru