Kombinierte Elektronenbeugungs- und "electron probe x-ray microanalysis"-Untersuchungen an Frühstadien der Zahnsteinbildung

H. J. Höhling, T. A. Hall, R. Mutschelknauss, J. Niem
1969 Zeitschrift für Naturforschung. B, A journal of chemical sciences  
1) From new calculus-formations, deposited on Mylar foils within 4 - 12 days, ultrathinsections were taken after alcohol-fixation. In the electronmicroscopical microanalyzer of Duncumb (5) we carried out Ca/P-measurements as well as relative-quantitative Ca- und P-determinations in microareas of mineralized and unmineralized tissue. 2) From the same person, 1 - 12 days after cleaning of the teeth, we also took the calculusformations, transfered them on Nylon foils, evaporated them with Al and
more » ... them with Al and carried out full quantitative Ca- und P-measurements in a normal microprobe. Concerning 1) our Ca/P values of partly mineralized microareas of the 4 days-foil are in the range of 0.95 - 1,3 and are far below the Ca/P molar ratio of apatite (1,67). Also the Ca/P ratios of mineralized microareas of older formations (e.g. 12 days) were found to ly normally below apatitic. The Ca/P-ratios of the neighbouring unmineralized microareas normally were even below those of the mineralized ones and increased with increasing Ca- and P-enrichment in the tissue. From this we conclude that Ca-enrichment in the tissue is the important step leading to nucleation which causes a further P-enrichment above the normal P-level in the tissue (probably by binding of HPO4-groups). This conclusion is supported by most of the results of 2).
doi:10.1515/znb-1969-0115 fatcat:skutr5ubmzaozn2xhiqpugztkq