Lead and cadmium resistant bacteria isolated from industrial effluent

Umamaheshwari, Poonguzhali, Sivanandhan, Khusro, Ignacimuthu
2015 Journal of Chemical and Pharmaceutical Research   unpublished
Bacteria play a major role in the biogeochemical cycling of toxic heavy metals. Heavy metal resistant bacteria can be used as bioremediation agents. The aim of this present investigation was to determine the resistance property of bacteria isolated from industrial waste dumped soil samples, to heavy metals (Pb 2+ and Cd 2+) and some antibiotics. Of 29 isolates, only two bacterial strains were found to be highly resistant to lead and cadmium. Further, these two bacterial strains were checked for
more » ... ns were checked for resistance against heavy metals by culturing them in basal medium in which varying concentrations of heavy metals were incorporated. HML8 and HMC1 isolates showed resistance against Pb 2+ and Cd 2+ at all concentrations tested. The morphological and biochemical tests confirmed that the isolates belonged to Bacillus sp. Both the isolates showed similar pattern of growth at different temperature ranging from 25⁰C to 42⁰C. HMC1 showed better growth in the presence of high NaCl concentration but the same was unable to grow at high pH. On the other hand HML8 showed good growth in the basal medium at high pH. High salt concentration affected the growth of HML8 in the basal medium. Both the isolates also exhibited high tolerance to antibiotics such as Amoxicillin and Penicillin G. The results of the present study clearly suggest that both the strains can be studied and explored further for biotechnological applications especially for lead and cadmium detoxification.