The Presence and Role of the Tetrodotoxin-Resistant Sodium Channel Nav1.9 (NaN) in Nociceptive Primary Afferent Neurons

Xin Fang, Laiche Djouhri, Joel A. Black, Sulayman D. Dib-Hajj, Stephen G. Waxman, Sally N. Lawson
2002 Journal of Neuroscience  
This is the first examination of sensory receptive properties and associated electrophysiological properties in vivo of dorsal root ganglion (DRG) neurons that express the TTX-resistant sodium channel Na v 1.9 (NaN). Intracellular recordings in lumbar DRGs in Wistar rats enabled units with dorsal root C-, A␦-, or A␣/␤fibers to be classified as nociceptive, low-threshold mechanoreceptive (LTM), or unresponsive. Intracellular dye injection enabled subsequent immunocytochemistry for Na v 1.9-like
more » ... mmunoreactivity (Na v 1.9-LI). Na v 1.9-LI was expressed selectively in nociceptive-type (Cand A-fiber nociceptive and C-unresponsive) units. Of the nociceptive units, 64, 54, and 31% of C-, A␦-, and A␣/␤-fiber units, respectively, were positive for Na v 1.9-LI. C-unresponsive units were included in the nociceptive-type group on the basis of their nociceptor-like membrane properties; 91% were positive. Na v 1.9-LI was undetectable in A␦-or A␣/␤-fiber LTM units and in one C-LTM unit. Na v 1.9-LI intensity was correlated negatively with soma size and conduction velocity in nociceptive units and with conduction velocity in C-fiber units. There was a positive correlation with action potential rise time in nociceptive-type units with membrane potentials equal to or more negative than -50 mV. The data provide direct evidence that Na v 1.9 is expressed selectively in (but not in all) C-and A-fiber nociceptive-type units and suggest that Na v 1.9 contributes to membrane properties that are typical of nociceptive neurons.
doi:10.1523/jneurosci.22-17-07425.2002 pmid:12196564 fatcat:cwftg3pl7bg37neaz6gbfxrv74