Pharma Science Monitor 5(3), Jul-Sep 2014 DEVELOPMENT OF VALIDATED HPTLC METHOD FOR SIMULTANEOUS DETERMINATION OF LEVOFLOXACIN HEMIHYDRATE AND CEFPODOXIME PROXETIL IN SYNTHETIC MIXTURE AND TABLET DOSAGE FORM

Hemal Vaidya, Henal Chorawala, Zarna Dedania, S Vijyendra Swamy
unpublished
Simple, precise and accurate method is described for the direct determination of Levofloxacin hemihydrate and Cefpodoxime proxetil in combined dosage form without prior separation. The method was based on HPTLC separation of the two drugs followed by the densitometry measurements of their spots at 237 nm for both drugs. The separation was carried out on silica gel 60GF254 using mobile phase Butanol: Ethyl Acetate: hexane: triethylamine(2:6:4:0.1).The linearity for Levofloxacin hemihydrates and
more » ... efpodoxime proxetil were found to be be 125-750 ng/spot and 100-600 ng/spot with r 2 0.9989 and 0.9992. The R f values were 0.66 and 0.33 for Levofloxacin hemihydrates and Cefpodoxime proxetil respectively.Developed method has been validated according to ICH guidelines.Percentage recoveries were found to be 99.89-101.59% for Levofloxacin hemihydrate and 98.96-102.01% for Cefpodoxime proxetil respectively.The proposed method was applied successfully for the determination of the two drugs in synthetic mixture and pharmaceutical dosage form.
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