Transcriptomic analysis to elucidate the response of honeybees (Hymenoptera: Apidae) to amitraz treatment

Liang Ye, Peng Liu, Tengfei Shi, Anran Wang, Yujie Zhu, Lai Li, Linsheng Yu, Yulin Gao
<span title="2020-03-06">2020</span> <i title="Public Library of Science (PLoS)"> <a target="_blank" rel="noopener" href="" style="color: black;">PLoS ONE</a> </i> &nbsp;
Amitraz is an acaricide that is widely used in apiculture. Several studies have reported that in honeybees (Apis mellifera Linnaeus; Hymenoptera: Apidae), amitraz affects learning, memory, behavior, immunity, and various other physiological processes. Despite this, few studies have explored the molecular mechanisms underlying the action of amitraz on honeybees. Here, we investigated the transcriptome of honeybees after exposure to 9.4 mg/L amitraz for 10 d, a subchronic dose. Overall, 279
more &raquo; ... entially expressed genes (DEGs) were identified (237 upregulated, 42 downregulated). Several, including Pla2, LOC725381, LOC413324, LOC724386, LOC100577456, LOC551785, and P4504c3, were validated by quantitative PCR. According to gene ontology, DEGs were mainly involved in metabolism, biosynthesis, and translation. Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that amitraz treatment affected the relaxin signaling pathway, platelet activation, and protein digestion and absorption.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="">doi:10.1371/journal.pone.0228933</a> <a target="_blank" rel="external noopener" href="">pmid:32143212</a> <a target="_blank" rel="external noopener" href="">fatcat:2qzhvnj7abhvlppxxlsm5zhori</a> </span>
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