Dietary ecologic camelina oil-a beneficial source of n-3 PUFA in muscle tissue and health status in finishing pigs

Mihaela Habeanu, Veronica Hebean, Ionelia Taranu, Mariana Ropota, Nicoleta Lefter, Daniela Marin
2011 Romanian Biotechnological Letters   unpublished
The objective of this study was to highlight the positive effects of the dietary ecologic camelina oil providing n-3 PUFA on fatty acid (FA) composition of total lipids of Longissimus dorsi, LD and Semitendinosus, ST muscles as well as its effects on blood plasma composition in finishing pigs. The experiment was carried for 30 days on 20 Large White finishing pigs randomly assigned to two isoenergetic and isonitrogenous diets: the control group (sunflower oil diet) received a diet including
more » ... diet including conventional ingredients based on sunflower oil (containing predominantly linoleic FA (C18:2n-6 = 64.62%) and the experimental group with supplement in n-3 PUFA by addition of camelina oil receiving a diet including ecological ingredients. Gas chromatography was used to determine the detailed composition in FA in muscle tissues (Longissimus dorsi and Semitendinosus). At the end of feeding trial blood samples were aseptically collected by jugular venipuncture from 6 animals of each group in order to determine the chemical composition (glycaemia, lipids and protein profile). Plasma biochemical analyses were performed using Analyser BS-130. Our results showed that the incorporation of α linolenic FA into muscle tissue was significantly higher following the dietary addition of camelina oil whatever the type of muscle. (by a factor of 2.83 on LD muscle, P<0.0001, and 1.40 on ST muscle, P<0.02, vs. control group). The reduction of C18:2n-6 : C18:3n-3 ratio (3.44 times in LD muscle and 2.13 times in ST muscle, P<0.0001 vs. control group) was important. The addition of n-3 PUFA to the experimental diet showed an accumulation of long-chain FA (DHA) known for its beneficial effects on human health. In plasma, the higher content of n-3 PUFA in the camelina oil influenced particularly the lipid composition. The concentration of cholesterol and triglycerides was decreased. Our results confirm that the camelina oil by its content in n-3 FA, alters significantly the fatty acids composition in the muscle tissue. Moreover, the results obtained in this study could be used for a better understanding of the biochemical processes in finishing pigs for estimating their physiological status.