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Fluorescentin situhybridization (FISH) reveals the abundance and positioning of nucleic acid sequences in fixed samples and can be combined with cell segmentation to produce a powerful single cell gene expression assay. However, it remains difficult to label more than a few targets and to visualize nucleic acids in environments such as thick tissue samples using conventional FISH technologies. Recently, methods have been developed for multiplexed amplification of FISH signals, yet it remainsdoi:10.1101/401810 fatcat:yw6cotdxmnhixab3u2o2spzuqu