Changes in 1,25-(OH)2D3Synthesis and Its Receptor Expression in Spleen Cell Subpopulations of Mice Infected with LPBM5 Retrovirus

T. M. Nguyen, J. Pavlovitch, M. Papiernik, H. Guillozo, O. Walrant-Debray, C. Pontoux, M. Garabedian
1997 Endocrinology  
This study examines the influence of chronic retroviral infection of mice with a LPBM5 virus mixture on the paracrine system involving immune cells and 1,25-(OH) 2 D 3 in the spleen. Plasma ionized calcium, 25-(OH)D and 1,25-(OH) 2 D of infected mice were unchanged. In contrast, the specific binding of 1,25-(OH) 2 D 3 to spleen cytosol and the number of monocyte/macrophages expressing 1,25-(OH) 2 D 3 receptors (VDR) were markedly increased. The retroviral infection also influenced the local
more » ... enced the local production of 1,25-(OH) 2 D 3 in the spleen. It did not alter this production in monocyte/macrophages but increased that in isolated T cells. Isolated B cells in control mice did not produce 1,25-(OH) 2 D 3 , but they increased the ability of isolated T cells to produce this metabolite during coculture incubations. Infection altered this cell interaction as 1,25-(OH) 2 D 3 production in infected T cells decreased when these cells were cocultured with infected B cells. Thus, chronic retroviral infection alters both the local vitamin D metabolism and VDR expression by immune cells in mice. These findings suggest close local interactions between 1,25-(OH) 2 D 3 and immune system activation during retroviral infection. (Endocrinology 138: [5505][5506][5507][5508][5509][5510] 1997) LPBM5 MuLV
doi:10.1210/endo.138.12.5582 pmid:9389537 fatcat:gf3zsd3irbh7jducfjh53n2efq