The Effect of Isoflurane on Neutrophil Selectin and β2-Integrin Activation In Vitro

Lothar W. de Rossi, Nicola A. Horn, Wolfgang Buhre, Florian Gass, Gabriele Hutschenreuter, Rolf Rossaint
2002 Anesthesia and Analgesia  
Isoflurane is reported to reduce ischemia-reperfusion injury. Lower expression of CD11b may be responsible for attenuated postischemic neutrophil adhesion to vascular endothelium. However, neutrophil adhesion to vascular endothelium is a multistep process involving several selectins and ␤ 2 -integrins. Therefore, we assessed whether isoflurane affects the activation of the selectins P-selectin glycoprotein ligand-1 (PSGL-1) and L-selectin and the ␤ 2 -integrins CD11a and CD11b. Whole blood was
more » ... ncubated for 60 min with 0.5 or 1 minimum alveolar anesthetic concentration (MAC) isoflurane. After incubation, neutrophils were activated with N-formylmethionyl-leucyl-phenylalanine (FMLP) or phorbol-12myristate-13-acetate (PMA). Activation of adhesion molecules was evaluated via flow cytometry, and 1 MAC isoflurane reduced the expression of CD11a in the unstimulated samples. After stimulation with FMLP and PMA, shedding of L-selectin was lower in the presence of isoflurane. Furthermore, 1 MAC isoflurane reduced FMLP-induced activation of CD11a and CD11b compared with unexposed blood samples. These results demonstrate that isoflurane affects the activation of three adhesion molecules involved in the multistep process of neutrophil recruitment. First, isoflurane inhibits the activation of L-selectin, which mediates the neutrophil tethering and rolling on the vascular endothelium. Second, isoflurane attenuates the activation of both ␤ 2 -integrins-CD11a and CD11b-which mediate firm adhesion and transendothelial migration. (Anesth Analg 2002;95:583-7)
doi:10.1097/00000539-200209000-00017 pmid:12198042 fatcat:st3atsbyxvf5nktm7so5lc76xq