Fluorescence correlation spectroscopy (FCS) has emerged as an ultrasensitive technique for monitoring molecular dynamics operating at the single-molecule level. In association with two-photon excitation (TPE), this fluorescence emission analysis has opened new opportunities for the study of biological systems. Indeed, owing to the very weak concentration of fluorophores used and the small volumes probed, FCS allows non-invasive in vivo studies. We have recently demonstrated that FCS under TPE

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... n be applied successfully to characterize the penetration and diffusion capabilities of fluorescent nanoprobes inside microbial biofilms. The formation of biofilms is an https://www.cambridge.org/core/terms. https://doi.