Detection of autoantibodies by indirect immunofluorescence and related techniques: the pathologist´s view

Julius Rajčáni
2015 Medical Research Archives  
The increasing importance of autoimmunity based diseases attracts the interest of pathologists, for whom the wide acceptance of indirect fluorescent antibody (IFA) technique at autoantibody screening is a relevant diagnostic approach. The IFA method has gained perfection by introducing the so called diagnostic mosaics, consisting of various tissue sections (primate and/or mammalian in origin), of HEp-2 cells, of microdots composed of specific purified antigen(s) and/or of transfected HEK cells
more » ... xpressing the target autoantigens. As result of this, the IFA technique dominates not only in the detection of anti-nuclear antibodies (
doi:10.18103/mra.v2i1.330 fatcat:lirz4f35djd57hzthbfszaxtna