Sex Identification Using the ZFX and ZFY Genes in Common Marmosets (Callithrix jacchus)

2011 Experimental animals  
We investigated sex determination via the ZFX and ZFY genes using PCR-RFLP in the common marmoset. We designed a novel primer set to detect ZFX and ZFY. A 483bp band from the ZFX gene and a 471-bp band from the ZFY gene were amplified. Sequencing data of the products amplified from ZFX and ZFY showed the recognition sites of two restriction enzymes, DdeI and MseI, respectively. After digestion of the products using each enzyme, we found that the band patterns between females and males were
more » ... and males were different. PCR-based sex identification might provide a tool for further breeding studies and experimental embryological studies using marmosets.
doi:10.1538/expanim.60.417 pmid:21791881 fatcat:ilfp3vxh3zcrzksqhomfvhq5be