Objective: Preterm birth (PTB) and Preterm labour (PTL) (PTL is defined as 'regular uterine contractions, which result in cervical changes before 37 Week of pregnancy. The main cause of neonatal morbidity and mortality with 5-18% of pregnancies was the preterm labour. Interleukin-6 (IL-6) amniotic fluid is an important cytokine used to identify intra-amniotic inflammation and those at risk to imminent premature delivery in patients with elevated AF IL-6. However, results of the traditional

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... rement method (ELISA) are generally unavailable in time in order to inform care. The aim of this study was to establish if intraamniotic inflammation and/or infectious patients and those intended to spontaneous pre-term delivery among women with preterm labour and intact membranes can be identified through a point of care (POC) test or lateral-flow immunoassay for AF IL-6 concentration measurement. Methods: In this study, one hundred and thirty-six singleton pregnant women who had symptoms of premature labor and amniocentesis were included. At the time of diagnosis, amniocentesis was carried out. In this study, the determination of AF (white blood cells, gram stain) was happened. By the results of AF culture, microbial invasion of the amniotic cavity (MIAC) was defined. Enzyme Linked Immunosorbent Assay (ELISA) and the lateral flow based immunoassay used to determine AF IL-6 concentrations. Define AF ELISA IL-6 as ≥ 2600 pg/ml as the primary result for intra-amniotic inflammation Results: (1) The concentrations of AF IL-6 has been determined by a POC test with a specificity (91%), sensitivity (93%) and a positive likelihood ratio (10) where Intraamniotic inflammation identified with the threshold of 740 pg/ml; (2) The POC test and IL-6's ELISA are also used to identify patients at risk of acute placenta inflammation, MIAC and impending spontaneous preterm delivery. Conclusion: Intra-amniotic inflammation in women who present subsequently with spontaneous delivery before 34 week of gestation or those who will present with preterm and intact membranes can be identified by POC AF IL-6 test. It can be achieved within 20 minutes -this has important clinical implications and opens up opportunities for early diagnosis and treatment of intra-amniotic infection / inflammation.